Modification of an adenovirus major late promoter-binding factor during poliovirus infection
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چکیده
منابع مشابه
Protein factor(s) binding independently to two different regions of the adenovirus 2 major late promoter.
The protein factor(s) in a fraction from the HeLa cell nuclear extract required for specific in vitro transcription can specifically bind to adenovirus 2 major late promoter (Ad 2 MLP) DNA. We demonstrate by in vitro footprinting assay that there are two asymmetric protected regions covering the TATA box and the nucleotides upstream from the TATA box. In the coding strand, the DNAse I protected...
متن کاملHigh mobility group proteins 1 and 2 stimulate binding of a specific transcription factor to the adenovirus major late promoter.
High mobility group proteins 1 and 2 (HMGs 1 and 2) are abundant chromosomal proteins which are believed to be preferentially associated with regions of active chromatin. Our previous results have shown that HMGs 1 and 2 can significantly stimulate specific transcription in vitro from the adenovirus major late promoter. This stimulation is now shown to be due, at least in part, to the influence...
متن کاملA single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
A simple approach has been developed for the unambiguous identification and purification of sequence-specific DNA-binding proteins solely on the basis of their ability to bind selectively to their target sequences. Four independent methods were used to identify the promoter-specific RNA polymerase II transcription factor MLTF as a 46-kilodalton (kDa) polypeptide. First, a 46-kDa protein was spe...
متن کاملAdeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor.
Activity of the adeno-associated virus P5 transcriptional control region was found to be induced by adenovirus E1A gene products. A pair of adjacent sequence elements was found to mediate both basal and E1A-induced P5 activity. The first element is a binding site for the major late transcription factor (MLTF), a factor first identified on the basis of its binding to a specific sequence within t...
متن کاملReplication-induced stimulation of the major late promoter of adenovirus is correlated to the binding of a factor to sequences in the first intron.
The sequence requirements for transcriptional stimulation of the adenovirus major late promoter (MLP) by the products of the early transcription unit Ela and by the replication of viral DNA were analyzed by in vitro transcription. Sequences upstream of +33 are involved in the moderate Ela-responsiveness of the MLP, while sequences between +33 and +131 are required for its major replication-indu...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1989
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.63.9.3858-3864.1989